Life Science Research Professional 1 (1 year fixed term)

Company: Stanford Medicine
Location: Castro Valley
Posted on: January 11, 2022

Job Description:

Roger Kornberg's lab at Stanford is seeking a highly motivated research assistant (Life Science Research Professional 1) to join our team (1-year fixed-term position with a possibility for extension). Your research work will focus on protein expression and purification of the different components of the transcriptional machinery. In this role you will be working closely with scientists who are using structural biology to answer fundamental questions regarding gene expression and chromatin structure.

Your duties and responsibilities will include:

• Engineering recombinant protein in yeast, E.coli, or insect cell.
• Purification pf proteins at semi-preparative or preparative scale.
• Introducing modifications on proteins using different chemistries.
• Performing biochemical assays to test protein functions.
  Candidates should have a background in the biological or chemical sciences and laboratory experience with the following techniques:
  
  • standard molecular biology techniques including plasmid DNA transformation and propagation, PCR, mutagenesis, and cloning.
  • chromatography, specifically protein-based applications of ion exchange, affinity, and size exclusion chromatography/other size-based separation techniques.
    You will work directly with Dr. Rayees Mattoo. His research explores is focused on understanding the mechanism of RNA Polymerase II mediated (eukaryotic) transcription using biochemical and structural approaches.
    
    DESIRED QUALIFICATIONS :
    
    • Bachelor's or higher degree in biology, chemistry, or a related scientific field.
    • Experience in protein purification techniques including ion exchange, SEC, and affinity tag methods.
    • Experience in standard molecular biology techniques.
    • Ability to maintain detailed records of experiments and outcomes.
    • Experience in culturing bacteria and yeasts.
    • Experience with laminar flow hoods for culturing insect or mammalian cells.
      
      
      EDUCATION & EXPERIENCE (REQUIRED):
      
      Bachelor's degree in related scientific field.
      
      KNOWLEDGE, SKILLS AND ABILITIES (REQUIRED):
      
      • General understanding of scientific principles. Demonstrated performance to use knowledge and skills when needed.
      • Demonstrated ability to apply theoretical knowledge of science principals to problem solve work.
      • Ability to maintain detailed records of experiments and outcomes.
      • General computer skills and ability to quickly learn and master computer programs, databases, and scientific applications.
      • Ability to work under deadlines with general guidance.
      • Excellent organizational skills and demonstrated ability to accurately complete detailed work.
        
        
        PHYSICAL REQUIREMENTS*:
        
        
        • - Consistent with its obligations under the law, the University will provide reasonable accommodation to any employee with a disability who requires accommodation to perform the essential functions of his or her job.
          WORK STANDARDS:
          
          • Interpersonal Skills: Demonstrates the ability to work well with Stanford colleagues and clients and with external organizations.
          • Promote Culture of Safety: Demonstrates commitment to personal responsibility and value for safety; communicates safety concerns; uses and promotes safe behaviors bases on training and lessons learned.
          • Subject to and expected to comply with all applicable University policies and procedures, including but not limited to the personnel policies and other policies found in the University's Administrative Guide, http://adminguide.stanford.edu.
            
            
            Additional Information
            
            • Schedule: Full-time
            • Job Code: 4945
            • Employee Status: Regular
            • Pay Grade: E
            • Department URL: http://structuralbio.stanford.edu
            • Requisition ID: 91230As an organization that receives federal funding, Stanford University has a COVID-19 vaccination requirement that will apply to all university employees, including those working remotely in the United States and applicable subcontractors. To learn more about COVID policies and guidelines for Stanford University Staff, please visit https://cardinalatwork.stanford.edu/working-stanford/covid-19/interim-policies/covid-19-surveillance-testing-policy

Keywords: Stanford Medicine, Castro Valley , Life Science Research Professional 1 (1 year fixed term), Other , Castro Valley, California